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1

Identification of a Universal VHH Framework to Graft Non-canonical Antigen-binding Loops of Camel Single-domain Antibodies

Dirk Saerens, Mireille Pellis, Remy Loris, Els Pardon, Mireille Dumoulin, André Matagne, Lode Wyns, Serge Muyldermans, Katja Conrath

Journal:

Journal of Molecular Biology

Year:

2005

Language:

english

File:

PDF, 539 KB

english, 2005

2

Comparison of llama VH sequences from conventional and heavy chain antibodies

Khoa Bang Vu, Mehdi Arbabi Ghahroudi, Lode Wyns, Serge Muyldermans

Journal:

Molecular Immunology

Year:

1997

Language:

english

File:

PDF, 1.24 MB

english, 1997

3

Crystallization of glycosylated and nonglycosylated phytohemagglutinin-L

Dao-Thi, Minh-Hoa, Hamelryck, Thomas W., Poortmans, Freddy, Voelker, Toni A., Chrispeels, Maarten J., Wyns, Lode

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

1996

Language:

english

File:

PDF, 392 KB

english, 1996

4

Camel single-domain antibody inhibits enzyme by mimicking carbohydrate substrate

Thomas R. Transue, Erwin De Genst, Mehdi Arbabi Ghahroudi, Lode Wyns, Serge Muyldermans

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

1998

Language:

english

File:

PDF, 418 KB

english, 1998

5

The crystal structure of triosephosphate isomerase (TIM) from Thermotoga maritima: A comparative thermostability structural analysis of ten different TIM structures

Dominique Maes, Johan P. Zeelen, Narmada Thanki, Nicola Beaucamp, Marco Alvarez, Minh Hoa Dao Thi, Jan Backmann, Joseph A. Martial, Lode Wyns, Rainer Jaenicke, Rik K. Wierenga

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

1999

Language:

english

File:

PDF, 538 KB

english, 1999

6

The Activation of Electrophile, Nucleophile and Leaving Group during the Reaction Catalysed by pI258 Arsenate Reductase

Goedele Roos, Stefan Loverix, Elke Brosens, Karolien Van Belle, Lode Wyns, Paul Geerlings, Joris Messens

Journal:

Year:

2006

Language:

english

File:

PDF, 419 KB

english, 2006

7

The coordination of 5′-derivatives of adenosine to vanadate

Jens Richter, Dieter Rehder, Lode Wyns, A. Haikal

Journal:

Inorganica Chimica Acta

Year:

1995

Language:

english

File:

PDF, 358 KB

english, 1995

8

Thiol pegylation facilitates purification of chymopapain leading to diffraction studies at 1.4 Å resolution

Mohamed Azarkan, Dominique Maes, Julie Bouckaert, Minh-Hoa Dao Thi, Lode Wyns, Yvan Looze

Journal:

Journal of Chromatography A

Year:

1996

Language:

english

File:

PDF, 317 KB

english, 1996

9

An evolutionary scheme for the histones as derived from a study of internal repetitions and homologies among the different classes

Christian Wuilmart, Lode Wyns

Journal:

Journal of Theoretical Biology

Year:

1977

Language:

english

File:

PDF, 1.03 MB

english, 1977

10

Development of a downstream process for the isolation of Staphylococcus aureus arsenate reductase overproduced in Escherichia coli

Joris Messens, Gaynor Hayburn, Elke Brosens, Georges Laus, Lode Wyns

Journal:

Journal of Chromatography B: Biomedical Sciences and Applications

Year:

2000

Language:

english

File:

PDF, 300 KB

english, 2000

11

The fimbrial adhesin F17-G of enterotoxigenic Escherichia coli has an immunoglobulin-like lectin domain that binds N-acetylglucosamine

Lieven Buts, Julie Bouckaert, Erwin De Genst, Remy Loris, Stefan Oscarson, Martina Lahmann, Joris Messens, Elke Brosens, Lode Wyns, Henri De Greve

Journal:

Molecular Microbiology

Year:

2003

Language:

english

File:

PDF, 465 KB

english, 2003

12

The structures of rnase a complexed with 3′-CMP and d(CpA): Active site conformation and conserved water molecules

Ingrid Zegers, Dominique Maes, Minh-Hoa Dao-Thi, Lode Wyns, Freddy Poortmans, Rex Palmer

Journal:

Protein Science

Year:

1994

Language:

english

File:

PDF, 2.18 MB

english, 1994

13

A catalytic function for the structurally conserved residue Phe 100 of ribonuclease T1

Jan Doumen, Malgorzata Gonciarz, Ingrid Zegers, Remy Loris, Lode Wyns, Jan Steyaert

Journal:

Protein Science

Year:

1996

Language:

english

File:

PDF, 3.15 MB

english, 1996

14

Consistency matrices: Quantified structure alignments for sets of related proteins

Ivo Van Walle, Ignace Lasters, Lode Wyns

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

2003

Language:

english

File:

PDF, 610 KB

english, 2003

15

Purification, crystallization, and preliminary X-ray studies on the rhizome lectin from stinging nettle and its complex with NN′N″-triacetylchitotriose

Dr. Remy Loris, Minh Hoa Dao Thi, John Lisgarten, Lode Wyns

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

1993

Language:

english

File:

PDF, 358 KB

english, 1993

16

Investigation of the functional interplay between the primary site and the subsite of RNase T1: Kinetic analysis of single and multiple mutants for modified substrates

Jan Steyaert, Abdel Fattah Haikal, Lode Wyns

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

1994

Language:

english

File:

PDF, 589 KB

english, 1994

17

Structural analysis of two crystal forms of lentil lectin at 1.8 Å resolution

Remy Loris, David van Overberge, Minh-Hoa Dao-Thi, Freddy Poortmans, Norbert Maene, Lode Wyns

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

1994

Language:

english

File:

PDF, 1.38 MB

english, 1994

18

Crystallographic structure of metal-free concanavalin A at 2.5 Å resolution

Julie Bouckaert, Remy Loris, Freddy Poortmans, Lode Wyns

Journal:

Proteins: Structure, Function, and Bioinformatics

Year:

1995

Language:

english

File:

PDF, 1.52 MB

english, 1995

19

Expression inE. coliand purification of the active autoprotease P20 of classical swine fever virus

Gaëtan Muyldermans, Ma Concepcion Gabriel, Raymond Hamers, Lode Wyns

Journal:

Year:

1996

Language:

english

File:

PDF, 585 KB

english, 1996

20

The monosaccharide binding site of lentil lectin: an X-ray and molecular modelling study

Remy Loris, Florence Casset, Julie Bouckaert, Jurgen Pletinckx, Minh-Hoa Dao-Thi, Freddy Poortmans, Anne Imberty, Serge Perez, Lode Wyns

Journal:

Glycoconjugate Journal

Year:

1994

Language:

english

File:

PDF, 1.11 MB

english, 1994

21

Kinetics and active site dynamics ofStaphylococcusaureusarsenate reductase

Joris Messens, José C. Martins, Elke Brosens, Karolien Van Belle, Doris M. Jacobs, Rudolph Willem, Lode Wyns

Journal:

JBIC Journal of Biological Inorganic Chemistry

Year:

2002

Language:

english

File:

PDF, 283 KB

english, 2002

22

Sequence-specific1H,15N and13C resonance assignments of the 23.7-kDa homodimeric toxin CcdB fromVibrio fischeri

Michal Respondek, Lieven Buts, Natalie De Jonge, Sarah Haesaerts, Remy Loris, Laurence Van Melderen, Lode Wyns, Klaus Zangger

Journal:

Biomolecular NMR Assignments

Year:

2009

Language:

english

File:

PDF, 216 KB

english, 2009

23

1H,13C and15N assignments of a camelid nanobody directed against human α-synuclein

Anneleen Vuchelen, Elizabeth O’Day, Erwin De Genst, Els Pardon, Lode Wyns, Mireille Dumoulin, Christopher M. Dobson, John Christodoulou, Shang-Te Danny Hsu

Journal:

Biomolecular NMR Assignments

Year:

2009

Language:

english

File:

PDF, 203 KB

english, 2009

24

Purification of an oxidation-sensitive enzyme, pI258 arsenate reductase from Staphylococcus aureus

Joris Messens, José C. Martins, Ingrid Zegers, Karolien Van Belle, Elke Brosens, Lode Wyns

Journal:

Journal of Chromatography B

Year:

2003

Language:

english

File:

PDF, 1.85 MB

english, 2003

25

The affinity of the FimH fimbrial adhesin is receptor-driven and quasi-independent of Escherichia coli pathotypes

Julie Bouckaert, Jenny Mackenzie, José L. De Paz, Beatrice Chipwaza, Devapriya Choudhury, Anton Zavialov, Karin Mannerstedt, Jennifer Anderson, Denis Piérard, Lode Wyns, Peter H. Seeberger, Stefan Osc

Journal:

Molecular Microbiology

Year:

2006

Language:

english

File:

PDF, 498 KB

english, 2006

26

Location of the three disulfide bonds in an antimicrobial peptide from Amaranthus caudatus using mass spectrometry

MOUNIR EL BOUYOUSSFI, GEORGES LAUS, PATRICIA VERHEYDEN, LODE WYNS, DIRK TOURWE, GEORGES VAN BINST

Journal:

Chemical Biology & Drug Design

Year:

1997

Language:

english

File:

PDF, 297 KB

english, 1997

27

Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin

Lieven Buts, Abel Garcia-Pino, Anne Imberty, Nicolas Amiot, Geert-Jan Boons, Sonia Beeckmans, Wim Versées, Lode Wyns, Remy Loris

Journal:

Year:

2006

Language:

english

File:

PDF, 995 KB

english, 2006

28

Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallograph

Zegers, Ingrid, Loris, Remy, Dehollander, Gillis, Haikal, Abdel Fattah, Poortmans, Freddy, Steyaert, Jan, Wyns, Lode

Journal:

Natural Structural Biology

Year:

1998

File:

PDF, 704 KB

1998

29

10.1038/nsb1001-843

Zegers, Ingrid, Martins, José C., Willem, Rudolph, Wyns, Lode, Messens, Joris

Journal:

Natural Structural Biology

Year:

2001

Language:

english

File:

PDF, 3.52 MB

english, 2001

30

Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme

Desmyter, Aline, Transue, Thomas R., Ghahroudi, Mehdi Arbabi, Dao Thi, Minh-Hoa, Poortmans, Freddy, Hamers, Raymond, Muyldermans, Serge, Wyns, Lode

Journal:

Natural Structural Biology

Year:

1996

File:

PDF, 1.33 MB

1996

31

Structure of a membrane-based steric chaperone in complex with its lipase substrate

Pauwels, Kris, Lustig, Ariel, Wyns, Lode, Tommassen, Jan, Savvides, Savvas N, Van Gelder, Patrick

Journal:

Nature Structural and Molecular Biology

Year:

2006

Language:

english

File:

PDF, 247 KB

english, 2006

32

Letter to the Editor: 1H, 13C and 15N backbone resonance assignment of the arsenate reductase from Staphylococcus aureus in its reduced state

Doris M. Jacobs, Joris Messens, Rainer W. Wechselberger, Elke Brosens, Rudolph Willem, Lode Wyns, José C. Martins

Journal:

Journal of Biomolecular NMR

Year:

2001

Language:

english

File:

PDF, 59 KB

english, 2001

33

Camel heavy-chain antibodies: diverse germline VHH and specific mechanisms enlarge the antigen-binding repertoire

Nguyen, Viet Khong, Hamers, Raymond, Wyns, Lode, Muyldermans, Serge

Journal:

The EMBO Journal

Year:

2000

Language:

english

File:

PDF, 678 KB

english, 2000

34

Nonspecific base recognition mediated by water bridges and hydrophobic stacking in ribonuclease I from Escherichia coli

Sergio Martinez Rodriguez, Santosh Panjikar, Karolien Van Belle, Lode Wyns, Joris Messens, Remy Loris

Journal:

Protein Science

Year:

2008

Language:

english

File:

PDF, 800 KB

english, 2008

35

Single-domain antibody fragments with high conformational stability

Mireille Dumoulin, Katja Conrath, Annemie Van Meirhaeghe, Filip Meersman, Karel Heremans, Leon G.J. Frenken, Serge Muyldermans, Lode Wyns, Andre Matagne

Journal:

Protein Science

Year:

2002

Language:

english

File:

PDF, 264 KB

english, 2002

36

Encapsulation of therapeutic nucleoside hydrolase in functionalised nanocapsules

Gerard Huysmans, An Ranquin, Lode Wyns, Jan Steyaert, Patrick Van Gelder

Journal:

Journal of Controlled Release

Year:

2005

Language:

english

File:

PDF, 290 KB

english, 2005

37

Combining sites of bacterial fimbriae

Henri De Greve, Lode Wyns, Julie Bouckaert

Journal:

Current Opinion in Structural Biology

Year:

2007

Language:

english

File:

PDF, 1.89 MB

english, 2007

38

Toxin–antitoxin modules as bacterial metabolic stress managers

Lieven Buts, Jurij Lah, Minh-Hoa Dao-Thi, Lode Wyns, Remy Loris

Journal:

Trends in Biochemical Sciences

Year:

2005

Language:

english

File:

PDF, 608 KB

english, 2005

39

Legume lectin structure

Remy Loris, Thomas Hamelryck, Julie Bouckaert, Lode Wyns

Journal:

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

Year:

1998

Language:

english

File:

PDF, 1.32 MB

english, 1998

40

Novel structures of plant lectins and their complexes with carbohydrates

Julie Bouckaert, Thomas Hamelryck, Lode Wyns, Remy Loris

Journal:

Current Opinion in Structural Biology

Year:

1999

Language:

english

File:

PDF, 605 KB

english, 1999

41

Recognition of antigens by single-domain antibody fragments: the superfluous luxury of paired domains

Serge Muyldermans, Christian Cambillau, Lode Wyns

Journal:

Trends in Biochemical Sciences

Year:

2001

Language:

english

File:

PDF, 301 KB

english, 2001

42

A single-domain antibody fragment in complex with RNase A: non-canonical loop structures and nanomolar affinity using two CDR loops

Klaas Decanniere, Aline Desmyter, Marc Lauwereys, Mehdi A Ghahroudi, Serge Muyldermans, Lode Wyns

Journal:

Year:

1999

Language:

english

File:

PDF, 191 KB

english, 1999

43

Reconsidering the energetics of ribonuclease catalysed RNA hydrolysis

Stefan Loverix, Georges Laus, José C. Martins, Lode Wyns, Jan Steyaert

Journal:

Year:

1998

Language:

english

File:

PDF, 134 KB

english, 1998

44

Assembly of oligonucleosomes into a limit series of multimeric higher-order chromatin structures

Serge MUYLDERMANS, Ignace LASTERS, Raymond HAMERS, Lode WYNS

Journal:

Year:

1985

Language:

english

File:

PDF, 545 KB

english, 1985

45

Assembly of oligonucleosomes into a limit series of multimeric higher-order chromatin structures

Serge MUYLDERMANS, Ignace LASTERS, Raymond HAMERS, Lode WYNS

Journal:

Year:

1985

Language:

english

File:

PDF, 784 KB

english, 1985

46

Scatter analysis of discrete-sized chromatin fragments favours a cylindrical organization

Ignace LASTERS, Lode WYNS, Serge MUYLDERMANS, John P. BALDWIN, George A. POLAND, Colin NAVE

Journal:

Year:

1985

Language:

english

File:

PDF, 753 KB

english, 1985

47

Dissection of the ribonuclease T1 subsite : The transesterification kinetics of Asn36Ala and Asn98Ala ribonuclease T1 for minimal dinucleoside phosphates

Jan STEYAERT, Abdel F. HAIKAL, Patrick STANSSENS, Lode WYNS

Journal:

Year:

1992

Language:

english

File:

PDF, 523 KB

english, 1992

48

Complex of ribonuclease Sa with a cyclic nucleotide and a proposed model for the reaction intermediate

Jozef SEVCIK, Ingrid ZEGERS, Lode WYNS, Zbigniew DAUTER, Keith S. WILSON

Journal:

Year:

1993

Language:

english

File:

PDF, 58 KB

english, 1993

49

Complex of ribonuclease Sa with a cyclic nucleotide and a proposed model for the reaction intermediate

Jozef SEVCIK, Ingrid ZEGERS, Lode WYNS, Zbigniew DAUTER, Keith S. WILSON

Journal:

Year:

1993

Language:

english

File:

PDF, 483 KB

english, 1993

50

Functional Interactions among the His40, Glu58 and His92 Catalysts of Ribonuclease T1 as Studied by Double and Triple Mutants

Jan Steyaert, Lode Wyns

Journal:

Journal of Molecular Biology

Year:

1993

File:

PDF, 755 KB

1993